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While GLP-1 medications dont directly deplete nutrients, people eating less may develop low or borderline levels, particularly: Vitamin B12 (especially if also taking metformin) Vitamin D Iron (especially in menstruating women) These labs arent required for everyone

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METHOD DETAILS Sample preparation FOXO4 FHD (95-195) was subcloned into the pET His6 Tobacco Etch Virus (TEV) LIC cloning vector (2B-T, obtained from Scott Gradia, Addgene Plasmid #29666) and transformed into BL21 (DE3) cells