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In Vitro Skin Irritation Test HaCaT keratinocytes were cultured in DMEM containing 10% fetal bovine serum and 1% penicillin/streptomycin and were maintained under 37 C with humidified 5% CO 2

Because of this, dark spots or patches may appear on the lips from childhood (often as small brown or black dots)

Detection of LongR 3 -IGF-I, Des(1-3)-IGF-I, and R 3 -IGF-I using immunopurification and high resolution mass spectrometry for antidoping purposes

Cummings MA, Arias AW, Stahl SM

The underlying mechanism is potentially associated with the downregulation of HSP90 and P53