Biofilms were washed once with 1 PBS and stained with a live/dead stain (Bacterial Viability Kit, Molecular Probes, Thermo-Fisher Scientific, USA), followed by incubation in the dark for 45 min before imaging

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When PT141 binds to these receptors, it initiates a cascade of neurotransmitter responses that influence behavior, motivation, and mood, particularly via dopaminergic and hypothalamic pathways
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