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The open field apparatus used in this study was a square arena (41 41 cm) with walls (height: 30.5 cm) to prevent the animals from escaping

0:18We are doing a five milligram here

Nuclei were stained with DAPI (BD Biosciences 564907) diluted 1:2,000, and actin was stained with Alexa-568 phalloidin (Life Technologies, A12380) diluted 1:500

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It is the principal upstream regulator of GnRH secretion, integrating sex hormone feedback to determine when and how frequently GnRH and downstream LH is released

Higher doses do not increase glucose excretion proportionally and increase the risk of side effects without added benefit