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Cell viability was quantified using the Cell Counting Kit-8 (CCK8) assay

The drop containing cells was covered with a glass cover slip and left at 4C for 5 min

Modulation of antioxidant defence system for arsenic detoxification in Indian mustard

Grasso M, Bond GJ, Kim YJ, Boyd S, Matson Dzebo M, Valenzuela S

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Clinical trials have consistently demonstrated that this restoration leads to a dramatic, measurable reduction in specific biomarkers of systemic oxidative damage, such as TBARS (thiobarbituric acid reactive substances) and F2-isoprostanes, which are direct indicators of lipid membrane destruction