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2.4 Effect of Phi on Pc GKB4 growthdose-response curve analysis A total of 54 sterile 100 mL conical flasks, each containing 25 mL of RMM broth supplemented with 10 mM KH 2 PO 4 and Phi at concentrations of 0, 0.125, 0.25, 0.5, 0.75, 1, 1.25, 2.5, 5, 10, 20, 40, 60, 80, 100, 200, 500, and 1,000 g/mL in triplicates, were inoculated with 125 L of the above prepared inoculum