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IX-71 inverted microscope the pipette-to -bath DC resistance of patch electrodes ranged from 4.0 to 5.2 , and were filled with a internal solution of the following composition (in mM): 25 CsCl, 100 CSCH3O3S, 10 phosphocreatine, 0.4 EGTA, 10 HEPES, 2 MgCl 2) 0.4 Mg-ATP, and 0.04 Na-GTP

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Ethical Approval This study was deemed not to constitute research involving human subjects according to 45 Code of Federal Regulations 46.102(f) and deemed exempt from board oversight and from full review by the RTI International institutional review board

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