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Although they initially originated from the parent variant selected for the fastest growth on glucose-ammonia minimal medium, auxotrophs required threonine (strain 23), nicotinic acid (strain 122), or tryptophan (strains 160, 166, and 168) due to damage of some key metabolic genes

All protocols require informed consent, medical oversight, and realistic expectations about potential outcomes

In a region where iodine-containing food (such as seaweed) is common, as in Japan, excessive dietary iodine intake (1000 micro g/day or more) may cause transient hypothyoidism in patients with subclinical autoimmune thyroiditis

for extended storage, freeze at 20 C (4 F)

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Mild to severe lymphocytic-plasmacytic or lymphocytic gastritis may be present