A second ultracentrifugation was performed at 100,000 g for 1.5 h at 10 C
Mild taste for easy acceptance
Acknowledgements This work was supported by the National Natural Science Foundation of China (30600661, 30872708, 30801174, 30871191 and 30900622), the Scientific Research Foundation of the Health Bureau of Hunan Province (B2007058), the Natural Science Foundation of Hunan Province (05C0163) and the Post-doctorate Scientific Research Special-purpose Project of Hunan Province (2008RS4012)
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Temperature management is the critical variable