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52 In addition, most cancer cells are extremely vulnerable to glucose withdrawal, 53, 54, 55, 56, 57, 58, 59 a feature they owe to metabolic reprogramming, leading to constitutively active proliferation pathways and glucose addiction

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However, TK inhibitors face the problem that IGF-1R and IR are so similar

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Briefly, samples were deproteinized by the addition of sulfosalicylic acid, were then incubated for 30 min at 4C, and subsequently centrifuged for 20 minutes at 12,000 g