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After 4 and 8 weeks, the animals were sacrificed by an overdose of sodium pentobarbital (IV) and the articular cartilage on the femoral condyles in the right knee joint was opened for macroscopic evaluation

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According to the Arthritis Foundation and CDC resources, each pound of body weight translates to approximately 3-4 pounds of force across the knee joint during walking

10.3389/fnins.2019.00217

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These are administered by healthcare professionals according to NHS protocols