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The membrane was blocked with PBS containing 0.5% tween (PBST) and 5% milk, and probed overnight with affinity-purified anti-GspD or anti-PilQ in PBST plus 3% BSA

This helps limit oxidative damage from UV radiation and pollution, as concluded by the research 4

Shufeiya Recipe inhibits oxidative stress by activating SIRT3/FOXO3a to upregulate Mn-SOD and COX-1/2, and simultaneously activates PI3K/AKT/eNOS to promote NO/sGC/cGMP/PKG-mediated vasodilation, while inhibiting the Ras/MEK1/2/ERK1/2/c-fos pathway to reduce cell proliferation, thereby regulating oxidative stress and vascular remodeling (Jia et al., 2022

NAD+ (Nicotinamide Adenine Dinucleotide) is a coenzyme that plays a crucial role in cellular energy production and repair