These findings are consistent with previous studies suggesting that Mel and GSH may prevent structural damage in ovarian histoarchitecture by suppressing oxidative stress.10,11,15-18,21,22 Both Mel and GSH are key endogenous antioxidant molecules that neutralize free radicals and preserve tissue integrity by limiting oxidative stress-induced cellular damage.30,31 It has been previously reported that platinum-based chemotherapeutic agents such as carboplatin increase intracellular ROS production, thereby triggering lipid peroxidation (as indicated by elevated MDA levels), inflammatory responses (increased TNF- and IL-6), cellular damage, and apoptosis.2,7,8 In the current study, the increased immunoreactivity of Caspase-3 and NF-B, along with significantly elevated levels of TNF- and IL-6 in the CARB group, indicate that CARB may have simultaneously activated both apoptotic and inflammatory pathways
Briefly, the mice were anesthetized with an overdose of 1.25% tribromoethanol (0.2 mL/10 g body weight), and then euthanized by cervical dislocation in accordance with AVMA guidelines 26,27
doi:10.1073/pnas.1513973112 Underhill SM et al (2020): Amphetamine Stimulates Endocytosis of the Norepinephrine and Neuronal Glutamate Transporters in Cultured Locus Coeruleus Neurons
Moreover, both natural aging or HIV-1 infection cause changes in gastrointestinal tract, liver, and kidney function that collectively affect the pharmacology of administered drugs (McLean and Le Couteur, 2004)
She required peripheral parenteral nutrition and was transitioned to a feeding tube
PMID: 26701796
