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utilized ProtA-TurboID to discover the interaction between the FLYWCH/Zn-finger DNA-binding domain (FLYWCH1) and a subset of centromeres marked by H3K9Me3 in human cells [67]

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Briefly, 300 L reaction mixture was prepared by addition of 200 L of 0.1 M phosphate buffer (pH 6.5), 40 L of 1.5 mM l-tyrosine, 40 L of the selected compounds (101000 g/mL), 20 L of mh-Tyr (2000 U/mL) solution, and later incubated at 37 C for 10 min

At a glance, the two paths can look almost identical

Dubocovich, M