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Identification of cysteine residues involved in disulfide formation in the inactivation of glutathione transferase P-form by hydrogen peroxide

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10.1038/s41598-018-31270-3 Sci

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Reaction mixtures were then mixed with 2 l of 1 M TrisHCl (pH 8.3) and 1 U l 1 calf intestine phosphatase (Sigma) and incubated at 37 C for 30 min

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