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The first sample was dissolved in methanol with 0.1 mL of 50 % of the SSA and the second sample in an ammonium buffer pH 3.5 [H 2 O/ACN (90:10) (v/v) + 1 mM NH 4 (CH 3 COO)] with CH 3 COOH

References Brewer, K., & Grant, J

Class III peroxidases are located in extracellular space and are able to produce ROS in plants under certain conditions 7,32,33

Fluorescence imaging MCF-7 cells were incubated with either plain PLGA nanoparticles (0.1 mg/mL) or PLGA nanoparticles labeled with AuNCs (0.1 mg/mL PLGA, 0.8 g/mL AuNCs as gold) for 24 h in 10% FBS containing cell culture medium before imaging with confocal laser scanning microscope

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Besides the heme group, the active conformation of the enzyme consists of one tightly bound NADPH molecule in each subunit