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cDNA synthesis and PCR amplification Complementary DNAs (cDNAs) were synthesized at 42 C for 60 minutes with 3 L of RNA solution and 200 U/ L of Superscript II enzyme in a 20 L reaction mixture containing 0.15 g/ L random primers, first strand buffer 1X, 10 mM dithiothreitol (DTT), 0.5 mM dNTPs, and 2 U/ L RNAse inhibitor, then incubated at 94 C for 5 minutes

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Epithalon is used in controlled laboratory research based on the goals of the study, the research model, and the protocol being followed

E., Gurpide, E., Liu, R., & Anderson, K

Allergy Statement: Made without common allergens: milk/casein, eggs, fish, shellfish, tree nuts, peanuts, wheat, gluten, soybeans, and sesame

[DOI] [PubMed] [Google Scholar] 98.Wharton S, Lau DCW, Vallis M, et al