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Following incubation, cells were fixed with cold methanol and stained with crystal violet for 15 min at room temperature

NAD + /NADH is involved in this process within the mitochondria, where NAD + is reduced to NADH in the tricarboxylic acid (TCA) cycle and is subsequently oxidized to NAD + in the ETC for ATP generation

doi: 10.1089/ars.2016.6665 131 WangYHChangDYZhaoMHChenM

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Planning for subsequent years incorporates lessons learned during the first year to optimize protocols, timing, and integration with other wellness practices