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Although we narrowed down our inclusion criteria to only accept reports published in English, which may have improved quality, studies with optimistic results were still more favored for publication than those with negative findings
1,4 SUSTAIN 5: add-on basal insulin 1,10 In adult patients with type 2 diabetes on basal insulin metformin Mean change in A1C from baseline at Week 30 Patients with A1C 8% at screening had their background basal insulin dose reduced by 20% at the start of the trial to limit the potential risk of hypoglycemia
Membranes were subsequently stripped and incubated with anti-SNAP tag rabbit polyclonal antibody (P9310S, New England Biolabs, 1:1,000) followed by goat anti-rabbit HRP secondary antibody (ab6721, Abcam, 1:2,000) to detect total level of immunoprecipitated SNAP/FLAG-hGLP-1R per sample
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