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In the presence of 5.5 mM glucose, GLP- 1-mediated increments of [Ca 2+ ] i were reduced to approximately 20% after pre-treatment with 1 mM metformin (p 100 M metformin blocked this effect in the presence of 5.5 mM glucose, no effect of metformin was observed in the presence of 16.7 mM glucose (Figure 3(a))