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For BrdU staining, the primary BrdU antibody was added together with 0.5 U l 1 DNAse (Sigma, D5025) and 1 mM MgCl 2 (Sigma, M8266) in the blocking solution and incubated for 30 min at room temperature
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By deacetylating a variety of key proteins, SIRT1 enhances the antioxidant capacity of cells, reduces the production of ROS, and inhibits lipid peroxidation, thus alleviating the damage caused by ferroptosis to nerve cells (Xie et al., 2022
(2021) emphasized the significance of TB-500 in cardiac regeneration and repair, specifically highlighting its capacity to enhance cardiac function following hypoxia
Bonus: In addition to supporting muscle maintenance, creatine has neuroprotective effects, may support mood, and can improve cognitive performancea win for total wellness
