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Hum Reprod (2011) 26(11):3094101

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For immunodetection, the blots were incubated overnight in PBS containing 0.1% Tween-20 and 5% skim milk with the primary antibody

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These findings are supported by work from Liu et al who similarly observed increased expression of synaptic signalling proteins in the hippocampus, and reductions in A and tau burden in the frontal cortex and hippocampus of AD mice with 4-week progressive ladder-climbing RT (15 climbs, progression from 15%75% MCL) (Liu et al., 2020)