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Moreover, KO mice for liver IGF-1 gene developed muscle insulin resistance (it is important to mention that skeletal muscle myocytes express high number of IGF-1R), showing an increase in insulin concentrations and a substantial decrease in the insulin-induced autophosphorylation of the insulin receptor and IRS in skeletal muscle (being normal in liver and white adipose tissue)

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The dried-droplet method for inorganic matrices resembles that of organic matrices, wherein the inorganic matrix is either pre-mixed with the sample or sequentially layered during spot deposition