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doi:10.1038/cdd.2010.80

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Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin

While some pens may appear to have leftover medicine, this small amount is either used for flow testing (Ozempic) or is a slight manufacturing overfillnot intended for use as an extra dose

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