Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]
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1), with a WC mean reduction ranging from 6.4 to 14.6 cm 24,25,26,28,30,31
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The accumulation and misfolding of -syn is due in part to post-translation modification, particularly phosphorylation and nitration that can be exacerbated by oxidative stress (Brembati et al., 2023)