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pneumoniae , not only in the wt , but also in the gpoA, tpxD , and ahpD mutants, we used the same infection model for the A549 pneumocytes and RAW 264.7 macrophages, but with a treatment during infection of 5 mM NAC for 3 h for A549 cells, and 10 mM NAC for 1 h for RAW 264.7 cells

FMN also modulates epigenetic regulators like the histone methyltransferase KMT2B, indirectly influencing immune gene expression and neuroimmune balance, emphasizing the broader regulatory role of the RFK pathway

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SOD activity was measured in a reaction mixture containing 50 mM phosphate buffer (pH 7.8), 0.1 mM EDTA, 13 mM methionine, 75 M nitroblue tetrazolium (NBT), 2 M riboflavin, and 50 l enzyme aliquot 51