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After, neurons were incubated in blocking solution containing anti-guinea pig AF488 (1:200 dilution, minimal cross-reactivity Jackson ImmunoResearch cat no: 706-545-148) antibodies for 1 hour

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In organotypic hippocampal slice cultures (OHSCs), ferrostatin-1 can prevent neuronal death and reduce hemoglobin-induced iron accumulation, suggesting a pathogenic role for ferroptosis in cerebral hemorrhage [264]

Subsequently, added 60 L of 50% acetonitrile, vortexed with shaking at 1700 rpm for 10 minutes at 8 C, centrifuged at 15,000 g for 20 minutes at 4 C, and aspirated the supernatant and stored at 80 C until analysis