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Purified MDH (~1 mL) was dialyzed for 4 h in 1 L buffer 2 (20 mM MOPS-KOH pH 7.5, 150 mM NaCl) containing 10 mM EDTA followed by three times dialysis against 1 L of buffer 2

Proc Natl Acad Sci U S A (2009) 106:128590

Taking 3-5g daily without loading will reach the same levels, though it takes longer (about 3-4 weeks)

Impact of genetic subtypes of Prader-Willi syndrome with growth hormone therapy on intelligence and body mass index

1 killer of adults in industrialized nations

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