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Rabbit anti-GLP-1R antibody, mouse anti-glial fibrillary acidic protein (GFAP) and mouse anti-alpha smooth muscle actin ( -SMA) were from Abcam PLC (Cambridge, UK), Goat anti-type I collagen antibody from Southern Biotechnology (Birmingham, AL, USA), rabbit antibody against glyceraldehydes-3-phosphate dehydrogenase (GAPDH) from Trevigen (Gaithersburg, MD, USA) and anti-rabbit IgG Alexa488-conjugated antibody, anti-mouse-IgG-Alexa555-conjugated antibody, anti-goat-IgG-Alexa350-conjugated antibody and Hoechst33342 were from Invitrogen (Carlsbad, CA, USA)
It has been observed that the expression and deacetylase activity of SIRT3 are attenuated in vitiligo melanocytes
In addition, as shown in Fig
10.1172/JCI29069 45
For expressions of cyclin L2 alone, or cyclin L2 with one other protein subunit, the culture was inoculated with 1 mL of each virus encoding a protein (12 mL)