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Cell-free supernatants were collected and stored at -20C for the future assays and granulomas were harvested by adding ice-cold sterile 1x PBS to wells, followed by gentle scraping to achieve maximum recovery of granuloma lysates and stored at -20C

Events with amplitude 99% recovery of all proteins from the lysate

[8] This is particularly relevant because pulmonary fibrosis involves the same extracellular matrix dysregulation and chronic inflammation that GHK-Cu modulates in skin wounds, suggesting the peptide's effects are not tissue-specific

[PMID: 32554829]

Ingredients like milk thistle (silymarin) have been used for centuries to help protect liver cells from oxidative stress

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