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Because of its ease from the viewpoint of both the theory (flexible parts are digested, rigid parts are not) and practice (mix a query protein with a limited quantity of a protease, wait for a limited time, and separate the components of the resulting mixture) and usefulness of the generated information, this approach has a long history of useful utilization in protein research

Pathak K, Zhao Y, Calton EK, James AP, Newsholme P, Sherriff J, Soares MJ

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