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10.3390/biom13040680 82 Essam ElenanyY

The blood samples were diluted 1:2 in phosphate-buffered saline (PBS), layered onto a separation solution (Biocoll 1.077 g/ml, Biochrom AG, Berlin, Germany), and centrifuged (800 x g, 20 min, 20C, Multifuge X3 F2, Thermo Fisher Scientific, Dreieich, Germany)

Redox imbalance stress in diabetes mellitus: role of the polyol pathway

Oxygen polarography was performed at 37 0.001 C (electronic Peltier regulation), and oxygen concentration (M) and oxygen flux (pmol O 2 s 1 mL 1 ) were real-time recorded and corrected automatically for instrumental background by DatLab software (Oroboros Intruments, Innsbruck, Austria)

Interestingly, Zang et al

Mitochondria produce much of the energy used by heart cells