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For the DDB1-DDA1-DET1-COP1-Ube2e2(S76G-C139S)-c-Jun complex, the purified protein was prepared at a concentration of 0.225 mg/ml and applied to glow-discharged UltrAuFoil (1.2/1.3, 300 mesh) grid, with an incubation time of 10 sec at 4 C and 100 % humidity