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CaMKII-dependent diastolic SR Ca 2+ leak and elevated diastolic Ca 2+ levels in right atrial myocardium of patients with atrial fibrillation

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Thermal Shift assays Tryptophan fluorescence measurements were carried out using Prometheus Panta (NanoTemper Technologies)

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The accumulated Ca 2+ binds to the membrane, exposing specific buried thiols to the oxidants and impairing mitochondrial respiration, resulting in increased superoxide formation