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Subsequently, fertilized oocytes from each group were transferred individually to embryo culture droplets as SOF medium supplemented with 0.5 mM sodium citrate, 1 mM sodium pyruvate, 1% non-essential amino acids, 2% essential amino acids, 2 mM Gluta MAX , 2.8 mM myo-inositol and 4 mg/ml bovine serum albumin covered by mineral oil in a 35 mm sterile dish, followed by embryo culture in a CO 2 incubator (38.5C, 5%O 2 and 5%CO 2 ) (71)

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Typically, the frequency of GERP treatments in my protocol is three times per week: For 6-8 weeks, which is for mild cases (i.e

It may prevent the enzyme directly by obstructing the enzyme's active sites so it may not do its function, or it may do this indirectly by functioning as a potent anti-oxidant as well as cause the removal of hazardous radicals

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PMID: 36511281