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The NGs were treated 20 min at 37 C with 0.10.5 mg ml 1 collagenase Ia (Sigma), 0.40.6 mg ml 1 dispase II (Roche, Basel, Swiss), 15 g ml 1 DNase II type IV (Sigma), and 0.75 mg ml 1 bovine serum albumin (Sigma) in HKRB supplemented with 5.6 glucose

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This includes proteins in the nails

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injection of probes at NIR-I window, and (e) at NIR-II window

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Drug treatment In order to deactivate ERK1/2, prostate cells were pretreated with 10 M MEK1/2 inhibitor U0126 (MedChemExpress, China) for 24 h prior to plasmid transfection

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the refined peptide solution was transformed into salts using Waters 2545 RP-HPLC system with a chromatographic column of 50 x 250 mm reverse phase C18 column and 0.1% trifluoroacetic acid/acetonitrile as a mobile phase

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With MT-II coming in the form of an injection or nasal spray, Dr Yip said she was concerned it was experiencing a surge in popularity

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