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While we observed no autoproteolytic processing for recombinantly expressed and purified SnCE1 being acetylated at multiple lysine side chains, for SnCE1 C256A site-specifically acetylated at Lys78, Lys94, Lys98 (and Lys248) we observe an impaired SnCE1 autoproteolysis compared to non-acetylated SnCE1 C256A