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Reactions were performed with the qScript One-Step SYBR Green RT-qPCR, Low ROX Kit (Quantabio, Beverly, MA) in an AriaMx Real-time PCR System (Agilent, Savage, MD)

Since the pKa of Sec is ~ 5.2, the selenol (RSeH) of Sec exits in selenolate (RSe ) form under the physiological condition [8, 11,12,13,14,15,16,17,18]

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