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48 h after transfection, cells were washed twice with pre-warmed PBS and fixed with 4% paraformaldehyde (PFA) for 1015 min

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To view a copy of this license, visit About this article Cite this article Sato, K., Saigusa, D., Kokubun, T

As a glucagon-like peptide-1 (GLP-1) receptor agonist, it works by stimulating insulin secretion, suppressing glucagon, and slowing gastric emptyingmechanisms that do not directly affect urinary frequency

Proper technique is just as critical as accurate math