2.5 Quantification of oxidative phosphorylation and glycolysis The Seahorse XF96 Extracellular Flux Analyzer (Seahorse Bioscience, North Billerica, MA, United States) was utilized to measure real-time integrated cellular oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) as previously outlined ( 2.6 Western blotting (WB), real-time quantitative PCR (RT-qPCR) and reactive oxygen species (ROS) analysis WB analysis, RT-qPCR, and ROS analysis were performed as previously described (Supplementary Table S1
Since obesity is considered a chronic condition, many individuals may require continued care to achieve sustained weight loss and its associated health benefits 13
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While thirst itself isnt among the most frequently documented side effects, the gastrointestinal side effects that can lead to dehydration and subsequently trigger thirst are well-documented: Nausea: 12-24% of patients Diarrhoea: 12-22% of patients Vomiting: 2-13% of patients These digestive effects can cause fluid loss and trigger compensatory thirst, making it a secondary effect of the medications primary side effects
Mouse studies To investigate whether a GLP-1 stimulating bacterial strain identified in vitro could have an effect on metabolic disease markers in vivo , we performed a mouse study
