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The peptide enters the bloodstream directly, bypassing the digestive system

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Instead of using TFA for deprotection, a solution of HCl in 1,4-Dioxan ( c = 4 M, 5 equiv) was used, whereas 3i could be obtained after purification by column chromatography as a hydrochloride salt in a yield of 61.3 mg (0.4 mmol, 80%)

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Not everyone, however, has enough B12 in their bodies from diet alone, and this can lead to issues such as anemia