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In one embodiment, compound of formula- 13 is having purity of greater than 97% by HPLC

A volume of ice-cold acetonitrile equal to four times the sample serum volume was then added and the sample vortexed vigorously for 30 s

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Target cells were incubated with virus-containing medium and 2 g/mL polybrene (Sigma Aldrich) for 24 h, and then allowed recovery for 24 h prior to selection for transductants