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Hypoxia-inducible factor-dependent induction of myeloid-derived netrin-1 attenuates natural killer cell infiltration during endotoxin-induced lung injury
13(11) Alharbi SH
For VAPB WT versus P56S experiments, cells were co-transfected with 40 ng proinsulin-NLuc and 200 ng of either pEGFP-VAPB, pEGFP-VAPB-P56S [generated in house by site directed mutagenesis from pEGFP-VAPB with Fwd 5-CGATGATTCCGCTGTTGCTCCTCACACAGTACCTAC-3 and Rev 5- GTAGGTACTGTGTGAGGAGCAACAGCGGAATCATCG-3 mutagenesis primers and the Quick-Change Lightning kit (Agilent)], or pcDNA3.1+ as a control
W., & Sharma, B
The Three Amino Acids Necessary for Higher Glutathione Concentrations The three amino acids that are critical for supporting higher glutathione concentrations and assisting with proper function include: Glutamate is the first in the peptide sequence, and its side chain interacts with the cysteine moiety in a somewhat unusual peptide linkage, known as the -glutamyl bond
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