Sunny NE, Bril F, Cusi K
Dandona P, Ghanim H, Chaudhuri A
An aliquot from the wells containing the most active LacAnc100 and LaccID clones was inoculated in 3 ml of YPD (10 g l 1 yeast extract (Gibco, 212750), 20 g l 1 peptone (Gibco, 211677), 20 g l 1 d-glucose (Thermo Scientific, 410955000) and 25 g ml 1 chloramphenicol (Merck, C0378)) and incubated at 30 C and 225 rpm for 24 h
Yamanaka-Altenstein M, Rauch-Anderegg V, Heinrichs N
(2) TMAO group: TMAO (0.12%, Trimethylamine N-oxide dihydrate, Sigma) 2 was administered via drinking water for 56 days
In total, 300 islets from both experimental groups were frozen at 80C and subjected to posterior sonication (Sonic Dismembrator Model 100, Thermo Fisher Scientific, Waltham, MA, USA) in lysis buffer (in mmol l-1: HEPES, 50