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Real-Time PCR Total RNA was extracted from cells by acid guanidinium thiocyanate-phenol-chloroform extraction method, and complementary (c)DNA was synthesized using 1 g total RNA in a 20 L RT reaction mix containing 0.5 g/L of random primers, 0.1 mM dNTP, 0.1 M DTT and 5 first strand buffer

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Semaglutide works through GLP-1 alone, and tirzepatide combines GLP-1 with GIP, but neither touches glucagon

Caution should be taken with those that have megaloblastic anemia as B12 can reduce potassium levels in the blood