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Some basic laboratory measurements of glutathione status include: Whole blood glutathione (low) Gamma-glutamyl-transpeptidase (high) Urinary pyroglutamic acid (high) Furthermore, there are several functional markers which can be measured to assess riboflavin status, including direct measurement of red blood cell glutathione reductase activity: Urinary glutaric acid (high) Whole blood B2 Urinary adipic, suberic, ethylmalonic acids (high) Urinary succinic acid (high) can also be suggestive along with a few other organic acids Erythrocyte glutathione reductase activity (low) To summarize, the initial cellular processing of TTFD requires adequate levels of reduced glutathione