Reported applications include radioligand and fluorescent binding assays at CTR and AMY1 to AMY3, cAMP accumulation and other Gs-pathway readouts, receptor internalisation and regulation studies, and structural biology: Cao and colleagues (Nat Commun, 2025) reported cryo-EM structures of cagrilintide bound to calcitonin and amylin receptors with associated PDB depositions
One possible explanation for this difference is that nonsynaptic mechanisms of [Ca 2+ ] i rise, VGCCs, and release from internal stores were activated by the AP-only protocol sufficiently strong to produce calcium levels necessary for triggering plasticity all over the dendritic tree
Never reuse needles between vials cross-contamination risk jumps the moment a used needle moves to a new vial
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