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Positive deletion- and reconstituted- strains were screened by Southern analysis (Figure S1) and DIG-hybridisation probes were generated using primers ogliT-5 and ogliT-4

The product obtained is proportional to the amount of nitrite present in the sample (53)

Instead, its absorbed directly into the capillaries and enters systemic circulation

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The nasal spray format is currently being explored for its potential to facilitate efficient absorption pathways in research models under controlled conditions