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Trial support is deep: STEP (semaglutide, NEJM 2021), SURMOUNT (tirzepatide, NEJM 2022), and SURMOUNT-OSA (tirzepatide in obstructive sleep apnea, NEJM 2024)

These include high blood pressure, preeclampsia or eclampsia, uterine irritability, premature labor or preterm birth, and having a baby with low birth weight or fetal growth restriction

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For cell activity, the CCK-8 solution and corresponding medium were added to the wells, incubated at 28C for 2 hours, and absorbance was measured at 450 nm