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So that's a question, I think

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The extract was divided into four fractions: one for analysis by UPLC/MS/MS with positive ion mode electrospray ionisation (ESI) (positive mode), one for UPLC/MS/MS with negative ion mode ESI (negative mode), one for GC/MS and one for backup

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Molecular analysis revealed that, compared to controls, Usp2 OE mice exhibited enhanced induction of fibrotic (CCN2, FN1, COL1A1, and COL3A1) and ferroptotic (HMOX1) markers, alongside further suppression of GPX4 following UUO (Fig

The reaction was stopped by heat inactivation and centrifuged at 17,000 g for 5 min